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Protein in horse feed: crude protein, lysine and excess

How crude protein, digestible protein and lysine are related, why more protein does not automatically mean more muscle, and how to check a suspected excess.

Drawn grasses and flowering legumes next to a bowl of soya bean meal and a blank analysis sheet.
Explanatory illustration · Equivora

The short answer

For protein supply, what counts is the quantity, digestibility and amino acids of the total ration. Crude protein and small-intestine digestible protein are different reference values. Lysine is comparatively well researched; for other amino acids, knowledge gaps remain. Adding protein across the board does not lead to any proven gain in muscle 1.

Protein is the body's building material, not its fuel: it supplies the amino acids for muscle, connective and other tissue as well as for enzymes 2. Some of these amino acids are essential – the horse cannot make them itself and has to take them in through the ration. The most important of these in practice is lysine 1.

Two things are regularly confused when it comes to equine protein: how much protein is listed for the feed and how much of it actually arrives. That is exactly what this article is about.

Crude protein is not the same as usable protein

The classic Weende analysis gives “crude protein” as nitrogen × 6.25 – that is the total nitrogen in the feed, not the share the horse absorbs as amino acids 3. That is why, since the GfE (German Society of Nutrition Physiology) recommendations, equine protein has been assessed as small-intestine digestible (prececal digestible) crude protein, abbreviated in German as dvRP or pcvXP (pcd CP): only what is digested before the large intestine and absorbed as amino acids is available to the metabolism as building material 42.

The difference is large and depends on the type of feed. Prececal nitrogen digestibility is only around 27–41% for forage, but around 58–72% for concentrates 1. Most of the remaining forage nitrogen is broken down by microbes only in the large intestine and absorbed mainly as ammonia – the horse cannot make essential amino acids from it, and this nitrogen is lost again as urea 1.

How much does a horse need?

As with minerals, it is worth looking at both reference works here – with an important warning up front. The NRC gives the crude protein requirement (CP) for maintenance, graded by temperament: 1.08 g (calm), 1.26 g (average) and 1.44 g (active) per kg body weight per day 15. The GfE, by contrast, relates its requirement to metabolic body weight and to digestible protein: 3.0 g dvRP per kg BW^0.75 per day 467.

Reference value500 kg600 kg
NRC crude protein, calm540 g648 g
NRC crude protein, average630 g756 g
NRC crude protein, active720 g864 g
GfE dvRP (digestible)≈ 317 g≈ 364 g

Maintenance requirement per day. The NRC gives total crude protein, the GfE digestible protein (dvRP) – two different measures, do not offset them against each other.

The fact that the GfE gives “364 g” and the NRC “756 g” is not a contradiction and does not mean that the GfE assumes half the requirement. They are two different values: dvRP is a subset of crude protein – only the digestible share. You must therefore not convert the two figures into each other or average them 41. The maintenance share stays the same across all life stages; allowances for growth, pregnancy, lactation and work are added on top. The concrete calculation logic is set out in the article on calculating requirements.

Lysine, threonine and the question of what runs short first

A limiting amino acid is the one whose shortage is the first to limit how well the rest of the protein can be used – like the too-short roof beam that determines how high the whole roof can be. In pigs and poultry on cereal-based rations this is lysine, and this rule of thumb is often transferred to horses without question.

For horses, only one thing is initially robust: lysine is the only amino acid whose requirement is based on direct dose-response studies – nitrogen retention and growth 1. This requirement is 36 mg per kg body weight as a minimum and 54 mg per kg as an optimum, or alternatively around 4.3% of the crude protein requirement 1.

Body weightMinimum (36 mg/kg)Optimum (54 mg/kg)
500 kg18.0 g27.0 g
600 kg21.6 g32.4 g

Lysine requirement for maintenance per day, according to Mok & Urschel (2020) on an NRC basis.

For methionine and threonine, the German-language requirement standard does list tabulated values (in which threonine, as mentioned, is above lysine), but for both there is still no requirement figure backed by direct feeding studies in horses; threonine estimates range between 53 and 80% of lysine intake 16. Which amino acid actually limits first in the high-fibre equine ration has therefore not been conclusively settled. Only the practical consequence is clear: no effect on hoof, horn or coat can be derived from a single amino acid – such promises are not supported 1.

Quality before quantity

A survey of 201 horses described in the review found an average crude protein intake of 157% of the NRC recommendation. That is a finding for this group, not a statement that every horse gets too much protein. In a trotter study discussed there, the higher protein supply did not lead to greater gain, but it did lead to higher water intake, nitrogen excretion and urine volume 1.

Energy supply is part of the picture too: the review describes weight loss with energy deficiency despite protein intake. For checking a ration, this means assessing energy and usable protein together. Excesses are not a proven muscle-building strategy 1.

What forage and concentrates provide

Grass hay usually covers the maintenance protein requirement of adult horses; what matters, however, is the combination of quantity and digestibility. The crude protein content of grass hay varies greatly with botanical composition, cutting date and cut, and falls as the grass becomes over-mature – late, stalky hay is lower in protein and digestibility 21.

In terms of protein quality, legumes such as lucerne (alfalfa) and soya bean meal provide more and higher-quality protein than grasses; soya bean meal is the classic lysine-rich supplementary component – in the growth trials from which the lysine evidence comes, lysine was supplemented precisely in this way 12. This is exactly where targeted amino acid supplementation comes in: with rations that are short in digestible protein or lysine, for example with over-mature hay.

Can too much protein explain skin or stomach problems?

The nutrition sources cited here do not establish a causal link between a protein excess and sweet itch or gastric ulcers. A suspected oversupply needs a ration check; the symptoms need a veterinary investigation regardless. The skin guideline distinguishes between insect hypersensitivity and suspected food allergies, and the veterinary information on the stomach describes non-specific signs and a separate diagnostic process 89.

  • What do analysis values and actual intake amounts show?
  • Which protein reference value is being compared with which requirement?
  • Which veterinary findings exist independently of the feeding suspicion?

Requirement peaks and limits of interpretation

Horses need considerably more protein and lysine during growth, pregnancy and lactation – there is even a separate requirement formula for lactating mares, and the robust lysine evidence comes precisely from growth 1. These are also the groups for which no automatic recommendation is given: foals, young horses during rearing and pregnant and lactating mares belong in specialist and veterinary advice, because this is where requirements are highest and errors weigh most heavily.

What can be managed and documented is the intake through the ration – for that you need a feed or hay analysis 310. Plasma amino acid levels are a research method for estimating requirements, not a routine test for the yard 1.

Key takeaways

  • Crude protein and digestible protein requirements must not be offset against each other as if they were the same value 1.
  • Protein cannot make up for an inadequate energy intake at will 1.
  • A suspected protein excess is not a skin or stomach diagnosis 89.

Frequently asked questions

How can I tell if my horse gets too much protein?

Check feed intake, analysis values and requirement on the same reference basis. A single observation or a high crude protein percentage does not replace this balance; digestibility must also be taken into account 1.

Does more protein build more muscle?

That cannot be promised in general. The review describes no additional gain with protein excesses and highlights the importance of energy supply. More crude protein is therefore not a shortcut to building muscle 1.

Should I cut protein if my horse has sweet itch or stomach problems?

Do not base such a change on a suspicion. Sweet itch (summer eczema) and stomach findings need their own professional assessment; a feed list does not make these diagnoses 89. Have supply and symptoms checked separately.

Sources & studies

The content is based on the references listed below. It does not replace individual veterinary or feeding advice.

Show list of sources (10)
  1. Mok CH, Urschel KL (2020): Amino acid requirements in horses (Invited Review). Asian-Australasian Journal of Animal Sciences 33(5):679–695Review of amino acid requirements: in horses essentially only lysine is quantitatively established; there are no robust requirement figures for methionine/threonine
  2. Coenen M, Vervuert I (2020): Pferdefütterung. Begründet von H. Meyer. 6., aktualisierte Aufl., Georg Thieme Verlag, Stuttgart. ISBN 978-3-13-241778-6Standard reference work on equine nutrition in German-speaking countries; Table 4.17 mineral recommendations (recommended supply and minimum requirement), Table 4.18 derived sweat volumes per work level and weight class, Table 4.19 electrolyte performance requirement per sweat volume (in addition to maintenance requirement)
  3. Weender Futtermittelanalyse (Weender Analysensystem)Standard method for determining crude nutrients (crude protein, crude fibre, crude fat, crude ash) as the basis of feed and hay analyses
  4. GfE (2014) – Empfehlungen zur Energie- und Nährstoffversorgung von PferdenSpecialist reference in German-speaking countries (GfE, German Society of Nutrition Physiology), Committee for Requirement Standards
  5. NRC – Nutrient Requirements of Horses, 6th ed.International reference for the nutrient requirements of horses (National Research Council)
  6. Kienzle, Pankratz & Zeyner (2023) – Kompendium zur Rationsberechnung für PferdeAuthor group Pferdewissen (Kienzle/Pankratz, LMU Munich; Zeyner, University of Halle-Wittenberg); self-published, Oberschleißheim, ISBN 978-3-00-075668-9. No link – self-published, not freely accessible.
  7. Bayerische Landesanstalt für Landwirtschaft (LfL): Gruber Tabelle zur PferdefütterungPractical reference table with requirement and feed values based on the GfE recommendations; the basis of many ration calculations in German-speaking countries. Chapter 9.3, Table 39: most common poisonous plants for horses with habitat, toxicity and risk level (after Kamphues et al. 2014)
  8. Marsella R et al. (2023): Equine allergic skin diseases: Clinical consensus guidelines of the World Association for Veterinary Dermatology. Veterinary Dermatology 34(3):175–208Open-access consensus guideline (literature up to November 2022), DOI 10.1111/vde.13168: insect allergy, insect avoidance, diagnostic limits and weak evidence for feeding. Sweet itch is not equated with feed allergy or excess protein.
  9. Merck Veterinary Manual: Gastric Ulcers in HorsesStandard veterinary reference work: a definitive diagnosis requires gastroscopic visualisation of the empty stomach; clinical signs alone are not sufficient
  10. VDLUFA – Methodenbuch III (Futtermittel)Reference methods of agricultural testing laboratories (LUFA) for the analysis of feeds and forages